Immunoassay is a biochemical assessment that measures the concentration or presence of a macromolecule in a solution through the use of an antibody or antigen. If and when detected, the molecule is referred to as an 'analyte' and can be a range of different molecules, all of different forms and sizes, as long as the sufficient properties for the assay are created. Analytes in biological liquids such as urine are regularly measured using immunoassays for medical and research purposes.
Immunoassays come in a variety of different formats. Some assays are known as multi-step assays and are deployed - just as their name suggests - in multiple steps, with reagents being added and washed away or separated at various times. These are also commonly referred to as heterogeneous immunoassays or separation immunoassays.
Another type of immunoassay is known as a homogeneous or non-separation immunoassay. Here, the immunoassay is performed by simply mixing the reagents and sample, creating a physical measurement.
Calibrators are often used during immunoassays. These are solutions that are known to contain the analyte being tested, with the concentration of that analyte generally known. Being able to compare an assay's response to a real sample against the assay's response as a result of a calibrator enables researchers to interpret the signal strength in terms of the concentration or presence of an analyte in the sample.
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Immunoassays play a vital role in various bioanalytical settings such as food testing, security, clinical diagnostics and environmental monitoring. Therefore, talented individuals are required to fill important roles within various scientific establishments in order to continue the development of IAs. Immunoassays have come a long way over the last few decades, with technologies and increased understanding drastically shortening the IA procedure. With continued support, IA can progress even further.
We work with a number of the very best scientific organisations in the country, who are constantly looking to recruit the best talent available. With our help, we are able to pair these employers with gifted individuals who are ready to take the next big step in their career.
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